Fig. 6

NF-κB is involved in histone H4-induced HPSE expression. The phosphorylation of I-κBα protein (A) and nuclear translocation of NF-κB p65 (B) were measured by western blot analysis 24 h after HPMECs were challenged by histone H4 (15 mg/L). Blocking antibody (10 mg/L) against TLR1, TLR2, TLR4, or TLR6 was administered 2 h prior to histone H4 treatment. Two hours prior to histone H4 challenge PDTC (2.5, 5, 10, 20 μM) was added to the medium, and the change in HPSE mRNA was measured at 12 h (C) while HPSE protein at 24 h (D). The results of western blot analysis are representative of three similar samples. *p < 0.05, **p < 0.01 compared with the control group; #p < 0.05, ##p < 0.01 compared with the histone H4 group