Fig. 4

HPSE mediates histone H4-induced HS degradation. The inhibitive effect of siRNAs on HPSE transcription was measured by real-time qPCR (A, B). After HPSE underwent targeted knockdown with siRNA-377, HS degradation caused by histone H4 was determined by immunofluorescence (C) and flow cytometry (D). The results from immunofluorescence (original magnification ×400) and flow-cytometric analyses are representative of three similar samples. Arrowheads indicate the immunofluorescence staining of HS. *p < 0.05, **p < 0.01 compared with the control group; #p < 0.05, ##p < 0.01 compared with the histone H4 group