Fig. 2
Histone H4 promotes pulmonary HS degradation in mice. Twenty-four hours after the mice were challenged with Cl2 exposure (400 ppm, 30 min) or histone H4 injection (20 mg/kg), the changes in the pulmonary vascular glycocalyx were evaluated by endothelial HS staining (A) and circulating HS fragments (B). OGT2115 (15 mg/kg) was injected subcutaneously 6 h prior to Cl2 exposure or histone H4 injection. Enzymatic activity (C) and protein content (D) of plasma HPSE were measured by commercial kits. The results of immunohistochemistry are representative of three similar samples (scale bars, 100 μm). *p < 0.05, **p < 0.01 compared with the control group; #p < 0.05, ##p < 0.01 compared with the histone H4-injection group