Fig. 1
Role of histone H4 in Cl2-induced ARDS in mice. Histone H4 in BALF (A) and plasma (B) was measured 24 h after the mice were challenged with different concentrations of Cl2 (30 min). Blood gas (C), lung wet/dry mass ratio (D), pathological changes in lungs (E), and pathological score (F) were measured 24 h after Cl2 exposure (400 ppm, 30 min). Histone H4 (10 mg/kg) or anti-H4 antibody (20 mg/kg) was injected through the tail vein 1 h prior to Cl2 exposure. An HPSE inhibitor, OGT2115 (15 mg/kg), was injected subcutaneously 6 h prior to Cl2 exposure. Data are presented as mean ± SD (n = 6). The H&E-stained lung sections are representative of three similar samples (scale bars, 50 μm). *p < 0.05, **p < 0.01 compared with the control group; #p < 0.05, ##p < 0.01 compared with the Cl2-exposure group