Fig. 2

Characterization of ADSCs-Exo. The ADSCs-Exo observed by TEM showed a typical cup-shape (A, B). The representative nano-flow cytometer histogram of ADSCs-Exo (C). Western blot indicated that ADSCs-Exo were positive for CD9, CD81 and Flotillin, but they were negative for Calnexin (D). Flow cytometry showed that most of the ADSCs-Exo were positive for CD9 (E) and CD81 (F). G1 refers to ADSCs and G2 refers to ADSCs-Exo