Fig. 7

RELA-KD in Scgb1a1-expressing epithelial cells reduces CDE-induced TGFβ and myofibroblast expansion. Scgb1a1^CreER™^/+  × RelA^fl/fl mice were pre-treated with TX for 10 days as in Fig. 6 and challenged with/without CDE over 4 d, n = 5–8. A IFC. Sections were stained with anti-TGFβ1 Ab (red) and counterstained with DAPI (blue). Images obtained at × 20. B Quantitation of TGFβ1 staining. Relative fold changes in FI for each treatment group; *p < 0.01 compared to Control, t-test. C Bioactive TGFβ1 in BALF by ELISA. D IFC of dual stain between α-SMA (green) and COL1 (red) with blue DAPI blue nuclear staining. Representative images shown at × 20 magnification, with selected enhanced image of the epithelium dissecting the individual colors. E Quantification of αSMA + /COL1 + myofibroblasts