Fig. 4

The connection of TRB3 and MAPK signaling in PASMCs was explored by western blot analysis. A and C Western blot analysis of the phosphorylation of ERK, JNK, and p38 MAPK in PASMCs after TRB3 knockdown and overexpression. B Effect of ERK, JNK, and p38 MAPK inhibitors on TRB3 in hypoxic PASMCs by Western blot analysis. D Effect of ERK, JNK, and p38 MAPK inhibitors on TRB3 in PASMCs transfected with a lentiviral vector expressing TRB3 (lv-TRB3) by Western blot analysis. Data represent the mean ± SEM (n = 4). *P < 0.05 and **P < 0.01. ERK, extracellular signal‐regulated kinase; JNK, c‐Jun N‐terminal kinase; p38 MAPK, p38 mitogen-activated protein kinase (MAPK)