Fig. 3

TRB3 overexpression in PASMCs increased cell proliferation and migration but impeded cell apoptosis. A TRB3 overexpression was achieved by lentiviral transfection, and the efficiency was determined by Western blot analysis. B The CCK8 test was used to determine cell proliferation. C Crystal violet staining is presented at × 200 magnification for the Transwell assay, and the migrated cells were counted. D Cell apoptosis induced by TRB3 overexpression was detected using flow cytometry and the percentage of early apoptotic (Annexin V+/PI−) and late apoptotic (Annexin V+/PI+) cells was determined. Data represent the mean ± SEM (n = 4). *P < 0.05 compared to the normoxic PASMCs transfected with lv-NC. lv-TRB3, PASMCs transfected with a lentiviral vector containing TRB3 CDS; lv-NC (negative control), PASMCs transfected with an empty lentiviral vector