Fig. 4

IL-35 inhibited ECM-associated genes via competitively identifying STAT3 binding sites. A Lung epithelial cells were stimulated with IL-35 (5 ng/ml) for indicated time points, and phosphorylation of STAT1 and STAT4 were measured by WB. B Lung epithelial cells were co-stimulated with IL-35 (5 ng/ml) and IL-6 (10 ng/ml), and phosphorylation of STAT3 were measured by WB. C CHIP qPCR assay for evaluating the enrichment of STAT1 and STAT4 on the promoters of Col1a2 and Col3a1. Input was used as the relative normalization (n = 3). Data are representative of three independent experiments. Data are presented as mean ± SEM. Significance was determined by two-tailed Student’s t test. *P < 0.05