Fig. 6

APN upregulated PINK1-dependent mitophagy via the SIRT1 signaling pathway in PMVECs subjected to diabetic IR injury. A PMVECs were infected with SIRT1 siRNA (A–C) and a negative control (NC), and representative blots are shown. B PMVECs were infected with PINK1 siRNA (A–C) and a negative control (NC), and representative blots. C The mRNA expression of SIRT1 (mean ± SD). D The mRNA expression of PINK1 (mean ± SD). E The coimmunofluorescence of LC3-II (fluorescent green) and TOMM20 (fluorescent red) was assessed to measure the level of mitophagy in PMVECs in each group (scale bars, 20 μm). F Quantitative analysis of mitophagosome formation (mean ± SD). G Representative blots. H PINK1 expression (mean ± SD). I SIRT1 expression (mean ± SD). HF, high fat; HG, high glucose; SIR, simulated ischemia–reperfusion. (^ccP < 0.05 versus the HG/HG + SIR group, ^ddP < 0.05 versus the HG/HG + SIR + A group)