Fig. 4From: Adiponectin ameliorates lung ischemia–reperfusion injury through SIRT1-PINK1 signaling-mediated mitophagy in type 2 diabetic ratsCharacteristics of primary rat PMVECs. A Cobblestone appearance could be observed under an inverted microscope. B Cell nucleus DAPI staining merged with CD31 immunofluorescence staining. The nucleus showed blue fluorescence, and CD31 antigen expression showed red fluorescence in PMVECs. C Cell nucleus DAPI staining merged with FITC-conjugated lectin. The cytoplasm showed green fluorescence (scale bars, 50 μm). D The purity of PMVECs was about 88%. E PMVECs were deficient in CD45. F PMVECs-viability was measured by the CCK-8 kit (mean ± SD). G Cell apoptosis was determined through the flow cytometry in rat PMVECs (medians, interquartile range). eeP < 0.05 versus the Control (NG)Back to article page