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Fig. 4 | Respiratory Research

Fig. 4

From: Circ_PIP5K1A regulates cisplatin resistance and malignant progression in non-small cell lung cancer cells and xenograft murine model via depending on miR-493-5p/ROCK1 axis

Fig. 4

Downregulation of miR-493-5p counteracted the effects of si-circ_PIP5K1A on DDP-resistant NSCLC cells. Transfection of si-NC, si-circ_PIP5K1A, si-circ_PIP5K1A + anti-miR-NC or si-circ_PIP5K1A + anti-miR-493-5p was conducted in A549/DDP and H460/DDP cells. A the expression analysis of miR-493-5p was completed through RT-qPCR assay. B the determination of IC50 was performed through MTT assay. C–E the evaluation of cell proliferation and viability was carried out through colony formation assay (C) and MTT (D, E). F–H the examination of cell cycle (F, G) and apoptosis (H) was implemented through flow cytometry. I, J the assessment of cell migration (I) and invasion (J) was performed through transwell assay. **P < 0.01, ***P < 0.001

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Respiratory Research

ISSN: 1465-993X

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