Fig. 5
Blockade of EZH2 with 3-DZNeP or EZH2 siRNA inhibited STAT1 pathway in the M1-polarized MH-S cells while activated the STAT6 pathway and PPAR-γ in the M2-marophage polarization. A Expression levels of p-STAT1 and SOCS3 in the LPS-stimulated MH-S cells with different dosage of 3-DZNeP (0, 1, 2, 5, 10 µM) were quantified by densitometry and normalized using GAPDH. B Representative Western blot depicting either LPS or IL-4 stimulated MH-S cell lysates with different dose of 3-DZNeP (0, 1, 2, 5, 10 µM) probed for p-STA1, SOCS3, p-STAT6, SOCS1, PPAR-γ and GAPDH. C Expression levels of p-STAT6, SOCS1 and PPAR-γ in the IL-4 stimulated MH-S cells with different dosage of 3-DZNeP (0, 1, 2, 5, 10 µM) were quantified by densitometry and normalized using GAPDH. (* p < 0.05 vs. MH-S cells with 0  µM 3-DZNeP, #p < 0.05vs. MH-S cells with PBS determined by one-way ANOVA for multiple group comparisons). D Expression levels of p-STAT1 and SOCS3 in the LPS-stimulated MH-S cells pretreatment with siRNA targeting EZH2 or scrambled siRNA were quantified by densitometry and normalized using GAPDH. E Expression levels of p-STAT6, SOCS1 and PPAR-γ in the LPS-stimulated MH-S cells pretreatment with siRNA targeting EZH2 or scrambled siRNA were quantified by densitometry and normalized using GAPDH. F Representative Western blot depicting either LPS or IL-4 stimulated MH-S cell lysates transfected with siRNA targeting EZH2 or scrambled siRNA, then probed for p-STA1, SOCS3, p-STAT6, SOCS1, PPAR-γ and GAPDH. All data are expressed as mean ± SEM. (*p < 0.05 vs. SiNC or Si-EZH2 group, #p < 0.05 vs. LPS or IL-4 stimulated group, @p < 0.05 vs. SiNC + IL-4 or SiNC + LPS group determined by one-way ANOVA for multiple group comparisons)