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Fig. 3. | Respiratory Research

Fig. 3.

From: Inhibition of EZH2 prevents acute respiratory distress syndrome (ARDS)-associated pulmonary fibrosis by regulating the macrophage polarization phenotype

Fig. 3.

3-DZNeP suppressed M1 macrophage polarization while promoted M2 phenotype in MH-S cells. A The levels of IL-1β, IL-6, TNF-α were measured by ELISA in the supernatants of LPS stimulated MH-S cells and IL-10 levels were qualified by ELISA in IL-4 stimulated MH-S cells with different dose of 3-DZNeP (0, 1, 2, 5, 10 µM). B The mRNA expression of iNOS, Arg-1 and FIZZ-1 was measured by quantitative PCR. C Representative Western blot depicting MH-S cell lysates probed for EZH2, H3K27me3, iNOS, Arg-1, FIZZ-1 and GAPDH. D Expression levels of EZH2 and H3K27me3 were quantified by densitometry and normalized using GAPDH. E Expression levels of iNOS, Arg-1 and FIZZ-1 were quantified by densitometry and normalized using GAPDH. All data are expressed as mean ± SEM. (* p < 0.05 vs. MH-S cells with 0 µM 3-DZNeP, #p < 0.05vs. MH-S cells with PBS determined by one-way ANOVA for multiple group comparisons)

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Respiratory Research

ISSN: 1465-993X

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