Fig. 4

The effects of FAM13A on cell migration, invasion, and TGF-β1-induced EMT marker alterations in BEAS-2B cells. A and B BEAS-2B cells were transfected with lentiviral vectors expressing FAM13A, shRNA against FAM13A, or corresponding negative control and incubated for 48–72 h. Wound healing and Transwell chamber assays were performed to examine the roles of FAM13A in cell migration and invasion. Representative images are shown. Magnification × 100. C Upper panel: BEAS-2B cells were transfected with lentiviral vectors overexpressing FAM13A or negative control. Western blotting was performed at 48 h after transfection to measure the protein levels of FAM13A, E-cadherin, ZO-1, vimentin, α-SMA, and N-cadherin. GAPDH was used as an internal control. *P < 0.05 vs. control, ^#P < 0.05 vs. negative control. Lower panel: BEAS-2B cells were transfected with shRNA against FAM13A or negative control. Cells were stimulated with TGF-β1 (10 ng/mL) for 48 h. Western blotting was performed at 48 h after transfection to measure the protein levels of FAM13A, E-cadherin, N-cadherin, vimentin, and α-SMA. GAPDH was used as an internal control. Data are expressed as mean ± SD. n = 3 (technical replicates). The experiment was repeated three times. NC: negative control; LV: lentiviral vectors. *P < 0.05 vs. control. ^#P < 0.05 vs. negative control. ^&P < 0.05 vs. shFAM13A. ^$P < 0.05 vs. TGF-β1 + shFAM13A