Fig. 5
From: Lung macrophages drive mucus production and steroid-resistant inflammation in chronic bronchitis
Sputum macrophages from COPD chronic bronchitis patients are steroid resistant. A Effect of 50 nM dexamethasone (DEX) on TNFα protein levels in media from LPS stimulated CB sputum macrophages (COPD/CB SM) (n = 5) and alveolar macrophages (AM) from COPD or non-COPD patients (n = 4). Cells were pre-treated with compound or DMSO vehicle for 22 h and subsequently stimulated with LPS (100 ng/ml) for 6 h. Data are presented as mean ± standard deviation. B Linear regression correlation plot comparing sputum neutrophil differential counts (%) and sputum macrophage steroid responsiveness (% inhibition of TNFα at 50 nM dexamethasone)