Fig. 2

Knockdown of Serpinb10 expression reduced HDM-induced allergic asthma. a flow cytometry gating strategy for inflammatory populations in bronchoalveolar lavage fluid (BALF). Live cells can be separated in a CD11c^high and a CD11c^low cell population. Within the CD11c^high cell population, gate for macrophages (SiglecF^high and MHCII^low) and dendritic cells (SiglecF^low and MHCII^high). Within the CD11c^low cell population, gate for lymphocytes and non-lymphocytes based on CD3/CD19 and MHCII expression. Within the CD11c^low CD3/CD19^neg cell population, gate for neutrophils (Ly-6G^high) and eosinophils (Ly-6G^low, SiglecF^high). b counts for total cells, macrophages, lymphocytes, eosinophils and neutrophils in BALF. c lung sections were stained with hematoxylin–eosin. The arrows indicate the peri-bronchial infiltration of inflammatory cells; original magnification, × 400. d periodic acid-Schiff (PAS) staining of representative lung sections, arrows indicate PAS positive cells; original magnification, × 400. e the protein levels of IL-4, IL-5 and IL-13 in BALF were determined by ELISA. f HDM-specific IgE was measured in mouse sera using ELISA. Data in (b, e, f) were pooled from three independent experiments. n = 12–14. *, P < 0.05; **, P < 0.01; ***, P < 0.001