Fig. 5
TGF-β1 induces human lung fibroblast-mediated collagen gel contraction. Collagen gel contraction assays mediated by human lung fibroblasts were performed by stimulating collagen gels with TGF-β1 (2 ng/mL) for 0, 24, 48, 72 and 96 h, in comparison to untreated controls. Results are expressed as collagen gel size (percentage compared to 0 h). Statistical analysis was performed using two-way ANOVA then Bonferroni's multiple comparisons test (where * denotes a significant effect of TGF-β1 on human lung fibroblast-mediated collagen gel contraction (P < 0.05)). Data are mean ± SEM values from n = 9 human lung fibroblast primary cell cultures