Table 1 Primer sequences
From: Enhanced lung inflammatory response in whole-body compared to nose-only cigarette smoke-exposed mice
Target | Forward primer | Reverse primer | Accession ID |
|---|---|---|---|
RPL27 | 5′-GTCGAGATGGGCAAGTTCAT-3′ | 5′-TTCTTCACGATGACGGCTTT-3′ | NM_011289.3 |
MMP12 | 5′-TTTTGATGGCAAAGGTGGTA-3′ | 5′-GCCTCATCAAAATGTGCATC-3′ | NM_001320077.1 NM_001320076.1 NM_008605.3 |
TIMP1 | 5′-GTGGGAAATGCCGCAGAT-3′ | 5′-GGGCATATCCACAGAGGCTTT-3′ | NM_001044384.1 NM_011593.2 NM_001294280.2 |
CXCL1 | 5′-ACCGAAGTCATAGCCACACTC-3′ | 5′-TCTCCGTTACTTGGGGACAC-3′ | NM_008176.3 |
TNF-α | 5′-CTGAGTTCTGCAAAGGGAGAG-3′ | 5′-CCTCAGGGAAGAATCTGGAAAG-3′ | NM_013693.3 NM_001278601.1 |
IFN-γ | 5′-AGCGGCTGACTGAACTCAGATTGTAG-3′ | 5′-GTCACAGTTTTCAGCTGTATAGGG-3′ | NM_008337.4 |
IL-6 | 5′-TAGTCCTTCCTACCCCAATTTCC-3′ | 5′-TAGTCCTTCCTACCCCAATTTCC-3′ | NM_031168.2 |
IL-12p40 | 5′-GGACCAAAGGGACTATGAGAAG-3′ | 5′-CTTCCAACGCCAGTTCAATG-3′ | NM_001303244.1 |
IL-17 | 5′-GCTGGACCACCACATGAA-3′ | 5′-GCATCTTCTCGACCCTGAAA-3′ | NM_010552.3 |
IL-23 | 5′-GGCATCGAGAAACTGTGAGAA-3′ | 5′-AGCCACCCAGGAAAGTATAAT-3′ | NM_031252.2 |
IL-4 | 5′-CCCCAGCTAGTTGTCATCCTG-3′ | 5′-CAAGTGATTTTTGTCGCATCCG-3′ | NM_021283.2 |
IL-13 | 5′-CAGCCTCCCCGATACCAAAAT-3′ | 5′-GCGAAACAGTTGCTTTGTGTAG-3′ | NM_008355.3 |
IL-10 | 5′-GCTCCAAGACCAAGGTGTCTACAA-3′ | 5′-CCGTTAGCTAAGATCCCTGGATCA-3′ | NM_010548.2 |
TGF-β | 5′-CTGAACCAAGGAGACGGAATAC-3′ | 5′-GGGCTGATCCCGTTGATTT-3′ | NM_011577.2 |