Fig. 1

Experimental design. a To create an allergic sensitization model, mice were intranasally exposed to either phosphate-buffered saline (PBS) or house dust mite (HDM) extract, 5 days per week for 3 consecutive weeks. A single intranasal dose of pandemic H1N1 (pH1N1) was administered on day 0. Control animals instead received chorioallantoic fluid (CAF) from non-infected eggs as a vehicle control (n = 10 per group, except n = 9 for HDM + pH1N1). b Using the allergic sensitization and pH1N1 infection protocol, HDM-sensitized mice were intraperitoneally administered anti-IL-4Rα or IgG1 isotype control monoclonal antibodies through either a prophylactic (on days-12, -7, -2, 1, and 6 post-infection (p.i.)) or a therapeutic treatment strategy (on days 1 and 6 p.i.). Control animals were intranasally exposed to PBS or HDM, and received intraperitoneal administrations of the vehicle control, PBS