Fig. 3

Effect of SRT1720 on mitochondrial biogenesis and airway barrier function mediated by HDM. a BEAS-2B cells were treated with various concentrations of SRT1720 or vehicle for 6 h. Subsequently, the cells were stimulated with HDM (100 μg/ml) for 24 h and the cells were harvested. The expression of PGC-1α in the cells was analyzed by western blotting. (n = 3). **p < 0.01. b–d BEAS-2B cells were treated with vehicle or SRT1720 (1 μM) for 6 h. Subsequently, the cells were stimulated with HDM (100 μg/ml) for 24 h and the cells were harvested. The expressions of PGC-1α, TFAM, and E-cadherin in the cells were analyzed by western blotting (n = 8). e Transepithelial electrical resistance (TEER) in primary human bronchial epithelial cells (pHBEC) were measured (n = 4) after the cells were stimulated by HDM (100 μg/ml) or PBS for 24 h with or without SRT1720 (1 μM) using a Millicell ERS-2 voltohmmeter. All data are expressed as means ± SD. *p < 0.05, **p < 0.01