Fig. 2
From: Role of CXCL16 in BLM-induced epithelial–mesenchymal transition in human A549 cells
CXCL16 and its receptor CXCR6 were upregulated in BLM-induced EMT in A549 cells. A549 cells were treated with different concentrations of BLM for 24 h. CXCL16 (a) and CXCR6 (b) mRNA levels were detected by RT-PCR, the expression of sCXCL16 in the cell culture supernatant was detected by ELISA (c), and protein levels of CXCL16 (d) and CXCR6 (e) were detected by western blot (f). Expression of CXCL16 and CXCR6 were also detected by cellular immunofluorescence (g). BLM Bleomycin; GAPDH served as a loading control. *P < 0.05, **P < 0.01, ***P < 0.001. Significantly different versus 0 μg/ml. Data represented three independent experiments. Scale bar, 100 μm