Fig. 1
From: Bitter taste receptor agonists regulate epithelial two-pore potassium channels via cAMP signaling
Denatonium benzoate treatment increases the short circuit current (Isc) and transepithelial resistance of human sinonasal epithelial cells. Ussing chamber experiments examining Isc a, representative tracing; b summary data) and transepithelial resistance (Panel C, representative tracing, Panel D summary data) following treatment with increasing concentrations of denatonium benzoate (solid line) or sodium benzoate (dashed line). Characteristically greater negative Isc with subsequent treatment with 100 µM IBMX/10 µM Forskolin (I/F) that is blocked by 10 µM CFTRinh-172 treatment suggests the expected presence of CFTR in these cells. There is a significant increase in the Isc b (p < 0.0005) and transepithelial resistance d (p < 0.005) following treatment with increasing concentrations of denatonium benzoate but not with sodium benzoate. Shown in b and d are the individual data points, mean and standard error of the mean