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Fig. 3 | Respiratory Research

Fig. 3

From: Evaluation of secondhand smoke effects on CFTR function in vivo

Fig. 3

Secondhand smoke (SHS) decreases CFTR-mediated chloride ion transport in murine airways. a. Representative electrophysiology tracing of excised trachea from mice exposed to either control room air or SHS for 6 weeks and mounted in modified Ussing Chambers. b. Graph illustrates summary of CFTR-mediated short circuit currents (Isc) in response to stimulation with forskolin (10 μM) and IBMX (100 μM) under the conditions of basal-apical Cl ion gradient and inhibited Na+ absorption by epithelial sodium channel (ENaC) by amiloride (100 μM). c. Graph demonstrating bumetanide (10uM) inhibition of basolateral NKCC1, a Na+/K+/2Cl cotransporter, confirmed reduction in CFTR-dependent chloride ion transport. n = 6–8, *P < 0.05; **P < 0.005

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