Fig. 5
From: Periostin plays a critical role in the cell cycle in lung fibroblasts
Effect of periostin silencing on proliferation of IPF lung fibroblasts. a, b Expression of periostin RNA estimated by qRT-PCR or periostin protein estimated by the ELISA assay. The values were adjusted by GAPDH expression, and the folds for the values are shown for qRT-PCR in panel a. The complete results of nine clones of normal lung fibroblasts and five clones of IPF lung fibroblasts are depicted. Values are mean ± SD of three independent experiments. *P < 0.05. c The growth curves of IPF lung fibroblasts. The cells with (dashed line) or without (solid line) knockdown of periostin were plated at a density of 1.0 × 104 cells/well in 96-well plates. The cell numbers were evaluated at the indicated times. Values are mean ± SD of three independent experiments. *P < 0.05. d IPF lung fibroblasts were treated with 10 nM control (closed circle) or periostin (closed triangle) siRNA for 48 h. qRT-PCR for the indicated important cell cycle progression genes in periostin-silenced lung fibroblasts. The values were adjusted by GAPDH expression, and the folds for the values are shown. Values are mean ± SD of three independent experiments. *P < 0.05, NS: not significant