Fig. 7

MiR-130b and PTEN regulate α/γ-ENaC through PI3K/AKT pathway in AT2 cells. a Representative Western blot bands of p-AKT/AKT protein expression in AT2 cells that were transfected with miR-130b mimic/inhibitor. c Representative Western blot bands of α/γ-ENaC protein expression in AT2 cells that were treated with 10 µM PI3K/AKT inhibitor (LY294002) and/or siPTEN for 30 min. b, d and e Graphical representation of data obtained from Western blot assays for p-AKT/AKT and α/γ-ENaC. Bands were quantified using gray analysis (p-AKT/AKT, α-ENaC/β-actin, and γ-ENaC/β-actin). **P < 0.01, compared with Control or miR-130b mimic negative control (NC); ^&&P < 0.01, compared with siPTEN group, n = 4