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Fig. 1 | Respiratory Research

Fig. 1

From: Sub-ohm vaping increases the levels of carbonyls, is cytotoxic, and alters gene expression in human bronchial epithelial cells exposed at the air–liquid interface

Fig. 1

Sub-ohm vaping increases the levels of carbonyls in butter-flavored e-cig aerosols. The e-cig device was operated with three different atomizers (resistance of 0.15, 0.5 and 1.5 Ω) and different battery voltages (2.8, 3.8 and 4.8 V), which yield a total of 9 distinct heating conditions that were evaluated for e-cig aerosol nicotine and carbonyls content. Vaping was conducted under a topography profile of 3-s puff duration, and a 55-mL puff volume every 30-s. 10 puffs of each e-cig aerosol were collected on site at the Inhalation Research Facility at Louisiana State University and were shipped overnight on dry ice to Enthalpy Analytical, LLC for subsequent chemical analysis. a Concentrations (µg/puff) of nicotine, acetaldehyde, formaldehyde and acrolein in butter-flavored e-cig aerosols. b Concentrations (µg/puff) of nicotine, acetaldehyde and formaldehyde in cinnamon-flavored e-cig aerosols. Acrolein was below the limit of detection in the cinnamon-flavored e-cig aerosols. This initial profile screening was comprised of a one-time chemical analysis of 18 different e-cig aerosol samples

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