Fig. 3

H19 deficiency ameliorated bleomycin-induced pulmonary fibrosis in mice. a Representative images of hematoxylin–eosin (H&E) staining, masson’s trichrome staining, Sirius red staining and collagen I immunofluorescence staining of Sham Wt, Sham H19^−/−, Wt BLM and H19^−/− BLM mice. b Ashcroft score for Sham Wt (n = 10), Sham H19^−/− (n = 7), Wt BLM (n = 9) and H19^−/− BLM (n = 9) mice. c Quantifying collagen content with hydroxyproline assay in the lungs of Sham Wt, Sham H19^−/−, Wt BLM and H19^−/− BLM mice. Each group, n = 6–8. d Real-time PCR analysis for Tgfb1, Col1a1 and Acta2 in the lungs of Sham Wt, Sham H19^−/−, Wt BLM and H19^−/− BLM mice. Each group, n = 8–10. e Western blot analysis for collagen type I the lungs of Sham Wt, Sham H19^−/−, Wt BLM and H19^−/− BLM mice. f Quantification of the panel (e)