Fig. 3
From: Nrf2 protects against seawater drowning-induced acute lung injury via inhibiting ferroptosis
Nrf2 attenuated seawater-induced MLE-12 cell damage. Images (a) and quantification (b) of Nrf2 expression levels were analyzed by Western blot (n = 3). (c) Nrf2 was detected by immunofluorescence (Red: Nrf2, Blue: nucleus, Original magnification × 400. Bar = 20 μm). (d) Cell death was analyzed using Annexin V-FITC/PI staining with flow cytometry (n = 4). (e) Quantitative results of the percentage of cell death (n = 4). (f) CCK-8 assay for the determination of cell viability (n = 4). Data were presented as the mean ± SD. ⁎P < 0.05 vs. Control group, #P < 0.05 vs. SW group