Fig. 3

Pretreatment with AZM reduces the expression of SO₂ induced inflammatory interferon alpha responsive genes. a. Gene set enrichment analysis of positively correlated pathways with the highest normalized enrichment scores (NES) as a result of SO₂ challenging of the mice. Top three most positively correlated pathways were the inflammation related interferon alpha response, interferon gamma response and allograft rejection. FDR is false discovery rate and NOM p-val is nominal p-value. b. Volcano plot of differentially expressed genes comparing gene expression of non-exposed vs SO₂ exposed mice. Genes in the interferon alpha response pathway are colored green. c. Volcano plot of differentially expressed genes comparing gene expression of non-exposed vs SO₂ exposed mice that had been pre-treated with AZM. Genes in the interferon alpha response pathway are colored green. d. Heatmap of genes that were positively correlated to the interferon alpha gene set. On the left is shown the differential gene expression as a result of SO₂ exposure. On the right is shown the differential expression as result of SO₂ exposure in AZM pre-treated mice. e. Boxplot of selected genes in the interferon alpha gene set. All genes show a similar pattern of higher expression as a result of SO₂ exposure. The AZM pre-treatment of the unexposed as well as the SO₂ exposed resulted in similar expression levels as the unexposed, untreated mice. Boxplot made with the R package ggplot/geom. Data are represented as box and whiskers plots with boxes encompassing the 25th – 75th quartiles, and whiskers demonstrating 1.5 Interquartile range above and below. N = 3, except for placebo (N = 2). *** p ≤ 0.001