Fig. 4
From: HGF alleviates septic endothelial injury by inhibiting pyroptosis via the mTOR signalling pathway
c-Met/mTOR signalling pathway was measured by Western blot. EA.hy926 cells were stimulated with Lipo2000 (2 μL/mL) and LPS (2.5 μg/mL), followed by administration of HGF (25 ng/mL) with or without PHA-665752 (50 nM) or rapamycin (20 nM) immediately and 6 h later. a Met, p-Met-Tyr1234/1235, mTOR, p-mTOR-Ser2448, AKT and p-AKT-Ser473 in the treated EA.hy926 cell homogenates were measured by Western blot; b Quantification of relative expression, n = 3; *P < 0.05 compared with the tLPS group; #P < 0.05 compared with the tLPS+HGF group; PHA: PHA-665752, RAPA: rapamycin.