Fig. 2

Effects of ERC/mesothelin overexpression on cell adhesion to extracellular matrix (a), invasion/migration (scratch assay) (b, c), and cell proliferation (d, e) of H2452 cells treated with ERC/mesothelin-overexpressing or control vectors. b and d, cells observed under a phase-contrast microscope. c and e, quantification of invading/migrating (c) and proliferating (e) cells. Solid line, ERC/mesothelin (+) cells; dotted line, control cells. Numbers (%) in the vertical axis indicate the ratio of the area occupied by cells relative to the whole area of interest. In E, there was no significant difference between the two groups for all time points to 96 h. n.s. not significant; RFP, red fluorescent protein