Fig. 5

NF-κB aggravates LPS-induced inflammatory response in lung cells by promoting the expression of CXCR4 via down-regulating miR-194. Mice induced with LPS were injected with DMSO/PDTC alone or in the presence of NC /miR-194 antagomir. a Pathological changes of lung tissues in mice under different treatments observed by HE staining (200×); b W/D ratio of lung tissues in mice under different treatments; c miR-149 expression and mRNA expression of CXCR4 measured by RT-qPCR; d Expression of CXCR4 at protein level assessed by western blot analysis; e Activity of NF-κB detected by ELISA; f Expression of IL-1β, IL-6 and TNF-α in BALF of mice measured by ELISA. g Number of total cells, macrophages, lymphocytes and neutrophils in BALF of mice; h MPO activity of lung tissues in mice under different treatments. The data in the figures were all measurements, which were presented as mean ± standard deviation; data between different groups were compared by unpaired t-test. *, p < 0.05