Table 1 Nucleotide sequences of primers used for real-time PCR
From: TRIM72 promotes alveolar epithelial cell membrane repair and ameliorates lung fibrosis
Gene | Direction | Primer sequence | GenBank Accession # |
|---|---|---|---|
Trim72 | Forward Reverse | 5′- CCGGCAAGGCTAGATATCCA − 3′ 5′- CTTCTGGTCTGAGCACTCCA − 3′ | NM_001079932.3 |
a-SMA | Forward Reverse | 5′- GCTGGTGATGATGCTCCCA − 3′ 5′- GCCCATTCCAACCATTACTCC − 3’ | XM_006526606.2 |
Fn1 | Forward Reverse | 5’- GTGTAGCACAACTTCCAATTACGAA − 3′ 5′- GGAATTTCCGCCTCGAGTCT − 3’ | XM_006495697.3 |
Col1a1 Cdh1 Sftpc | Forward Reverse | 5’- CCAAGAAGACATCCCTGAAGTCA − 3′ 5′- TGCACGTCATCGCACACA − 3′ | NM_007742.4 |
Forward Reverse Forward Reverse | 5′- CAGCCTTCTTTTCGGAAGACT − 3′ 5′- GGTAGACAGCTCCCTATGACTG − 3′ | NM_009864.3 | |
5′- GAAGATGGCTCCAGAGAGCATC − 3′ 5′- GGACTCGGAACCAGTATCATGC − 3’ | NM_011359.2 | ||
Hopx | Forward Reverse | 5’- CAACAAGGTCAACAAGCACCC − 3′ 5′- GGCGCTGCTTAAACCATTTCT − 3’ | NM_175606.3 |
Aqp5 | Forward Reverse | 5’- ATGAATCGGTTCAGCCCCTC − 3′ 5′- TCGATGGTCTTCTTCCGCTC − 3’ | NM_009701.4 |
Gadph | Forward Reverse | 5’- CGACTTCAACAGCAACTCCCACTCTTCC − 3′ 5′- TGGGTGGTCCAGGGTTTCTTACTCCTT − 3’ | XM-017321385.1 |