Fig. 7

Co-culture CD11b⁺ myeloid cells and PASMCs. Co-culturing SOD₂-deficient-CD11b⁺ myeloid cells and PASMC cells using transwell plates (CD11b⁺ cells in the upper side; PASMCs in the lower side). a-c. Separation gain rate of CD11b⁺ cells analyzed by FACS. CD11b⁺ cells were separated from C57BL/6 and SOD₂^−/+ mice using magnetic beads. d. PASMCs (the lower side plates) were stained to measure the percentage of tunnel positive cells. e. Brdu positive staining in PASMCs in the four groups. f. mRNA expression of NLRP3 in CD11b⁺ cells. g. mRNA expression of NLRP3 in PASMCs. h. Secretion of IL-1β in the supernatant of the co-culture system. i. Secretion of IL-18 in the supernatant of the co-culture system. CON: Wild type mice in the normal air condition. CIH: Chronic intermittent hypoxia. WT: wild type mice. SOD₂^−/+: knock-down of SOD₂ gene heterozygous mice. N = 5. Comparisons between multiple groups were performed using ANOVA with the Bonferroni test. *p < 0.05, **p < 0.01, ***p < 0.001