Fig. 6
From: TBX2-positive cells represent a multi-potent mesenchymal progenitor pool in the developing lung
Premature S100A4 expression in TBX2-deficient bSMCs correlates with altered contraction in Tbx2cre/fl and Tbx2cre/+;HprtTBX2/y lungs. (a) Immunofluorescence staining of S100A4 (red) on frontal lung section of different stages of control and Tbx2cre/fl;R26mTmG/+ embryos. Stages are as indicated. Nuclei were counterstained with DAPI. Insets of overview images are magnified in the row below. (b, c) Diagrams of relative contraction intensity (b) and bar graphs of corresponding integral calculation (C, Average values: Control (left): 4.7 ± 2.3, Tbx2cre/fl;R26mTmG/+: 6.7 ± 2.8; Control (right): 5.4 ± 2, Tbx2cre/+;HprtTBX2/y: 3.3 ± 1.7) of the right main bronchi of lungs explanted at E12.5 and cultured for 36 h. Differences were considered significant with *p ≤ 0.05, ** p ≤ 0.01, *** p ≤ 0.005, using two-tailed Student’s t-test. Statistical values are provided in Table S2