Skip to main content
Fig. 2 | Respiratory Research

Fig. 2

From: Vitamin D deficiency exacerbates bleomycin-induced pulmonary fibrosis partially through aggravating TGF-β/Smad2/3-mediated epithelial-mesenchymal transition

Fig. 2

Vitamin D deficiency aggravates BLM-induced pulmonary inflammation and interstitial fibrosis. In the VDD group, animals were fed with vitamin D deficient fodder. In the Ctrl group, animals were fed with standard fodder. All animals except controls were injected with BLM (1.5 mg/kg) through the trachea. All animals were sacrificed 2 weeks after BLM. a Relative lung weight was measured. b-d Pulmonary histopathological injury and inflammation were evaluated by H&E. b A representative picture. Original magnification: 200×. c Pulmonary pathological scores. d Inflammatory cells in the lungs. e-f Col1α1 and Col1α2 were estimated with real-time PCR. (G-H) Collagen deposition was evaluated using Sirius red staining. g A representative picture. Original magnification: 200×. h The area of collagen deposition was calculated. All data were expressed as means ± SEM (N = 6). **P < 0.01, *P < 0.05

Back to article page

Respiratory Research

ISSN: 1465-993X

Contact us