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Fig. 8 | Respiratory Research

Fig. 8

From: Effects of cigarette smoke on barrier function and tight junction proteins in the bronchial epithelium: protective role of cathelicidin LL-37

Fig. 8

Effects of LL-37 on protein expression of TJ and AJ proteins in Calu-3 cells. Cells pretreated with or without 20 μg/ml LL-37 were exposed to 10% CSE for 12 h. a. Representative confocal immunofluorescence images of the effects of LL-37 on CSE-induced disruption of TJ and AJ proteins. Staining of occludin, ZO-1, claudin-3, and E-cadherin is shown in green, and DAPI staining is shown in blue. Scale bar, 10 μm. b. Representative western blots showing TJ and AJ proteins in Calu-3 cells. Band intensity was quantitated using densitometry. All results are representative of at least two independent experiments. Data represent means ± SEM (n = 4–6 per group). *p < 0.05 by one-way ANOVA

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