Fig. 1From: Nasal epithelial cells to assess in vitro immune responses to respiratory virus infection in pregnant women with asthmapNECs obtained from nasal brushings are true representatives of epithelial cells in the upper respiratory tract. (A) Primary nasal epithelial cells were obtained by nasal brushing from the anterior region of the inferior nasal turbinate. (B) Cellular content from the brushings was anlysed directly by cytospins, prepared using 100 μl aliquots of cell suspensions, stained with MGG. (C) pNECs obtained by nasal brushings were cultured in tissue culture flasks until confluent and produced the same “cobblestone-like” appearance as (D) pBECsBack to article page