Fig. 4

JVS-100 improves lung angiogenesis. a Lung sections stained with Von Willebrand Factor (green) demonstrating improved vascular density in hyperoxia-exposed pups treated with IT JVS-100. Original Magnification X100. Scale bars are 100 μm. b Hyperoxia exposure decreased vascular density but the administration of IT JVS-100 was associated with improved angiogenesis (P < 0.05; * RA-PL vs hyperoxia-PL or hyperoxia-JVS-100; **hyperoxia-PL vs hyperoxia-JVS-100; N = 10 animals/group). c Increase in lung VEGFR-2 expression in hyperoxia-exposed JVS-100 treated pups. A representative Western Blot is shown in the lower panel with VEGFR-2 expression normalized to β-Actin (P < 0.05; * RA-PL vs hyperoxia-PL or hyperoxia-JVS-100; **hyperoxia-PL vs hyperoxia-JVS-100; N = 6 animals/group) (d) Increased tubule formation 20 h after plating recombinant SDF-1 treated hyperoxia-exposed HPMEC on matrigel. Original Magnification X 100. Scale bar is 50 μm. Increase in the length (e) and number of cord-like structures (f) per high power field (HPF) in hyperoxia-exposed recombinant SDF-1 treated HPMECs (P < 0.05; *RA vs hyperoxia-control; ** hyperoxia-control vs hyperoxia recombinant SDF-10 ng/ml or hyperoxia recombinant SDF-100 ng/ml; all experiments were performed in triplicate). RA is room air and O₂ is hyperoxia