Fig. 2

Influence of digitoxin on NFκB signaling and cytokine/chemokine secretion from CF cells. a Western blot experiments demonstrate that digitoxin stabilizes IκBα in the presence of TNFα (n = 3 biological replicates per condition; representative blot is displayed). b Digitoxin suppresses baseline and TNFα-activated NFκB-driven luciferase expression (numbers of biological replicates per condition are indicated in parentheses). (C-E) Digitoxin suppresses GM-CSF (c), IL-6 (d), and IL-8 (e) secretion under baseline and TNFα-activated conditions (n = 3; digitoxin-treated conditions are compared against respective non-TNFα-treated or TNFα-treated controls). Abbreviations: C (Control, no additions), E (ethanol), Dig2 (2 nM digitoxin), Dig10 (10 nM digitoxin), and Dig25 (25 nM digitoxin). Significant pairwise two-tailed t-test results are also displayed: * p < 0.05; ** p < 0.01; *** p < 0.001