Fig. 1

LncRNA SNHG14 is up-regulated in ALI models and its knockdown protects against LPS-induced inflammation. A, expression of lncRNA SNHG14 in MH-S cells treated with LPS or PBS determined by RT-qPCR, * p < 0.05 vs. the PBS + MH-S group (MH-S treated with PBS), n = 10. B, expression of lncRNA SNHG14 in lung tissues from mice with ALI induced by LPS or mice treated with normal saline determined by RT-qPCR, * p < 0.05 vs. the normal group (mice treated with normal saline), n = 10. C, representative micrographs showing localization of lncRNA SNHG14 in MH-S cells identified by FISH (400 ×). D, expression of lncRNA SNHG14 after SNHG14-ASO transfection determined by RT-qPCR, * p < 0.05 vs. the NC-ASO group (MH-S cells transfected with NC-ASO). E, TNF-α and IL-6 levels in the MH-S cell supernatant after lncRNA SNHG14 silencing determined by ELISA, * p < 0.05 vs. the NC-ASO group (MH-S cells transfected with NC-ASO). F, the MH-S cell viability after lncRNA SNHG14 silencing measured by CCK-8 assay, * p < 0.05 vs. the NC-ASO group (MH-S cells transfected with NC-ASO). The statistical values were measurement data and expressed as mean ± standard deviation. Unpaired t-test was used for comparisons between two groups. Statistical analysis in relation to time-based measurements within each group was realized using two-way ANOVA with non-repeated measure. The experiment was repeated 3 times independently