Fig. 2

mtROS increases in IPF AMs. a Representative histograms of freshly isolated BALF cells from normal and IPF subjects labelled with CD45-FITC and either MitoSOX Red or Propidium Iodide were analysed by flowcytometry. Alveolar macrophages/monocytes were selected (see Additional file 1: Figure S1d for population analysis) and percentage of MitoSOX positive cells and mean fluorescence intensities (red outlined histograms) were analysed relative to CD45-FITC labelled populations (grey histograms). b and c Median and interquartile range of mean fluorescence intensities (MFI) and percentages of MitoSOX RED positive macrophages from Control (n = 15) and IPF (n = 43) patients (patients characteristics Additional file 2: Table S4). d Median and interquartile range of percentages of Propidium Iodide positive macrophages of matched IPF and normal samples. e-h Correlations of mean fluorescence intensities (MFI) of MitoSOX RED positive macrophages from IPF patients with CPI, DLco%, TLC% and age. (Data represented as median with interquartile range*p < 0.05,*** = p < 0.001, Mann-Whitney test, r = Spearman’scorrelation coefficient)