Fig. 5

MLN8237 combined with radiation treatment suppress tumor growth in vivo. a Combination of MLN8237 and radiation prolongs tumor growth delay in H460 xenograft model. After 6 to 8 days cell injection, mice have palpable subcutaneous tumor were treated with vehicle control, MLN8237 (30 mg/kg for 30 days), radiotherapy (2 Gy daily for 5 consecutive days), or combined MLN8237 and radiotherapy (mice were irradiated 1 h after MLN8237 treatment with 2 Gy daily for 5 consecutive days). Tumor growth delay as defined by the number of days required to reach a tumor volume of 1000 mm³ was measured. b Representative images of IHC staining for cleaved caspase 3. The magnification of the black box is 200X, the red box is magnified field of the black box (400X). c Staining for active caspase 3 was performed to measure apoptosis and the number of positive cells was scored and graphed by averaging three repeated experiments; *p < 0.05, ** p < 0.01