Fig. 3

AT2 and AT1 marker expression in 3D aggregate cultures is regulated by Wnt ligands. a SPC is down- regulated by all Wnt4, Wnt5a and Wnt7a. Wnt7a upregulated the AT1 marker AQP5 (n = 3). b 3D lung aggregate tissue sections were stained with anti-AQP5 antibody (red), while nuclei were stained with DAPI (blue). Size bar is 50 μm. Representative of biological repeats, n = 3. c Densitometry of AQP5 staining (n = 3). Independent t-test, * p < 0.05. d 3D lung aggregate tissues were treated with Wnt7a ligands then sectioned and stained with anti-beta-catenin (pseudo-green) and anti-phospho-beta-catenin (red) antibody, respectively, while nuclei were stained DAPI (blue). Size bar is 50 μm. Representative of three independent biological repeats (n = 3). e Densitometry of beta-catenin and phospho-beta-catenin staining (n = 3, respectively). Mann-Whitney test * p < 0.05, ** p < 0.01, *** p < 0.001