Fig. 6

IFN-γ and PFD reverse the effects of TGF-β1 on MMP and TIMP release. NHLFs were treated with INF-γ [10–30 ng/ml], PFD [300–500 μg/ml] or a combination of IFN-γ/PFD in the presence of TGF − β1 [2.5 ng/ml] for 3 days. Conditioned media was examined for MMP-2 using gelatin zymography, while MMP-1, MMP-3, MMP-7, TIMP-1 and TIMP-2 were examined using Western blotting with β-tubulin as a loading control. Data following densitometric analysis with a representative zymogram of MMP-2 (a) and blots for MMP-1 (b), MMP-3 (c), MMP-7 (d), TIMP-1 (e) and TIMP-2 (f) are shown. Data are presented as the mean ± SEM of 3 independent experiments, with each experiment run in duplicate. ^**P < 0.05 vs. TGF-β1 and ^## P < 0.05 vs. TGF-β1+ vehicle [DMSO]