Fig. 5

IFN-γ and PFD synergistically downregulate TGF − β1-induced collagen expression and release. NHLFs and IPF fibroblasts were treated with INF-γ [10–40 ng/mL], PFD [300–500 μg/ml] or a combination of IFN-γ/PFD in the presence of TGF − β1 [2.5 ng/ml] for 3 days. The upper panel shows COL1A1 gene expression measured by quantitative RT-PCR from NHLFs (a) and IPF fibroblasts (b). Data for the relative quantification of each target gene are plotted as 2^-ΔΔCT (mean fold change) using the YWHAZ housekeeping gene as a control. The lower panel shows the collagen concentration in the conditioned media from NHLFs (c) and IPF fibroblasts (d) cultured for 3 days as measured by Sircol assay. Data are presented as the mean ± SEM of 3 independent experiments, with each experiment run in duplicate. ^*P < 0.05 vs. TGF-β1 and ^# P < 0.05 vs. TGF-β1+ vehicle [DMSO], respectively