Fig. 2

Effects of ectonucleoside triphosphate phosphohydrolase-7 (ENTPD7) silencing on A549 and SM-MES-1 cells. a, b, f, g ENTPD7 mRNA and protein expression levels were detected by quantitative real-time polymerase chain reaction (qRT-PCR) and Western blot. GAPDH was used as an internal control. All PCR reactions and protein assays were performed in triplicate. c, h Cell counting kit-8 (CCK-8) assay was performed to tested cell viability. d, i The crystal violet staining was used to test the cell proliferation ability. Pale violet staining represented a low proliferation, and dark violet staining meant high proliferation. e, j Apoptosis rates were detected using flow cytometry. A549 and SM-MES-1 cells were stained with Annexin V-FITC and PI. ^{^}P < 0.05, ^{^^}P < 0.01, versus control group; ^*P < 0.05, ^**P < 0.01, versus vector group