Fig. 1

Recombinant expression of GSTCD in multiple cell types does not identify GST activity Levels of GST activity are determined through fluorescence measurements (mU/ml) and expression is shown relative to EV. Graphs show median and interquartile range data analysed using Kruskal-Wallis test. a GST activity in CHO-K1 cells stably transfected with GSTCD variant 1 (T1), GSTCD variant 2 (T2) or empty vector (EV) showing no increased activity above the EV control (n = 4). b HASM cells transiently transfected with EV control, T1, T2, GSTM5 positive control (M5) and transfection reagent only (TRO). The relative GST activity was shown to be significant between the EV and M5 (p = 0.002,) however the two isoforms of GSTCD did not show any increased GST activity (n = 4). c HBEC cells transliently transfected with the plasmids showed no significant increases in GST activity over the EV (n = 4)