Table 1 Primer and probe sequences used for the negative strand HRV-16 RT-PCR reactions
From: Rhinovirus replication and innate immunity in highly differentiated human airway epithelial cells
Name | Purpose of oligonucleotide | Sequence |
|---|---|---|
Neg-Tag-cDNA | Chimeric RT primer | 5′- ATCAGCGATGCCGAACGTAT GGCAGCATGGGCAACCT-3′ |
Neg-HRV16-For | Real-time PCR Forward Primer | 5′-TGCTGATGCAATACTCAAAAAGG-3’ |
Tag-Rev | Real-time PCR Reverse Primer | 5′- ATCAGCGATGCCGAACGTAT -3’ |
Neg-Probe | Real-time PCR Probe | 5’FAM-TGAAAAGCGAGGGA-MGB3’ |